Table 1.

Experimental combinations and outcomes for HCFs and iPSC-cFbs.

Cell type	Substrates (E)	Drug treatments	Treatment duration	Outcome
HCF	3 GPa, 25 kPa, 2 kPa	10 ng/ml TGF-β, 30 nM SD208, 10 μM SB431542	2–4 days	Proliferation: CyQUANT; RT-PCR: α-SMA, collagen 1; IF: α-SMA; western blot: α-SMA, collagen 1
hiPSC-cFb	3 GPa	10 ng/ml TGF-β, 30 nM SD208, 10 µM SB431542	2–4 days	Proliferation: CyQUANT; RT-PCR: α-SMA, collagen 1, collagen 3; IF: α-SMA

α-SMA alpha-smooth muscle actin, E elastic modulus, HCF human cardiac fibroblast, IF immunofluorescence, hiPSC-cFb human induced pluripotent stem cell-derived cardiac fibroblast, TGF-β transforming growth factor-beta, RT-PCR real-time reverse transcriptase polymerase chain reaction.