Table 3.
Target | Company, catalog# | Host | Concentration |
---|---|---|---|
Glyceraldehyde 3-phosphate dehydrogenase | CST, D16H11 | Rabbit | 1:10,000 |
α-Smooth muscle actin | CST, D4K9N | Rabbit | 1:1000 |
Collagen 1 | Abcam, ab34710* | Rabbit | 1:1000 |
Rabbit IgG | Li-Cor 800CW 926–32211 | Goat | 1:10,000 |
*ab34710 anti-collagen 1 antibody has affinity to both α1 and 2 subunits. Two bands are seen at ~ 130 and 170 kDa. Both subunits were quantified and expressed as a total expression relative to GAPDH loading control.