Table 4.
Myofibroblast markers on soft culture plates by RT-PCR and western blot.
| RT-PCR | Gene of interest | TGF-β mean fold change versus control | P | |
|---|---|---|---|---|
| 25 kPa | ACTA2 | 1.2 ± 0.6 | 0.9 | |
| COL1A1 | 1.0 ± 0.1 | 0.9 | ||
| 2 kPa | ACTA2 | 0.4 ± 0.2 | 0.6 | |
| COL1A1 | 0.5 ± 0.1 | 0.8 |
| Western blot | Protein of interest | Control (POI/GAPDH) | TGF-B (POI/GAPDH) | P |
|---|---|---|---|---|
| 25 kPa | α-smooth muscle actin | 107.2 ± 6.8 | 168.7 ± 26.5 | 0.2 |
| Collagen 1 | 101.3 5.5 | 97.8 7.1 | 0.7 | |
| 2 kPa | α-smooth muscle actin | 96.6 ± 3.5 | 130.2 ± 29.4 | 0.3 |
| Collagen 1 | 142.2 ± 52.8 | 135.9 ± 53.8 | 0.9 |
Data presented as mean ± S.E.M. t test of TGF-β treated cells versus vehicle control. Western blot data presented as a ratio of protein of interest (POI) and GAPDH as a loading control.