Figure 1.

LNP characterization

(A) Representative cryo-electron microscopy image of LNP encapsulating hOTC mRNA. LNPs were homogeneous and had an electron-dense core. (B) Representative ex vivo luciferase imaging of organs of a mouse 24 h after intravenous injection of Fluc-mRNA/LNP at 1.0 mg/kg. (C) Quantified fluorescence of these organs. Values are expressed as means ± SD with individual values. n = 3. (D) Western blotting of hepatic hOTC proteins induced by hOTC-mRNA/LNP injection. Female BALB/c mice were intravenously injected with hOTC mRNA only, Fluc-mRNA/LNP, or hOTC-mRNA/LNP at 1.0 mg/kg, or saline. Liver specimens were sampled the next day and subjected to western blotting. Heat shock protein 90 (HSP90) proteins were used as an internal control. n = 3/group.