Fig. 5.

Role of NOX4 on TGF-β-induced cell migration and Epithelial-Mesenchymal Transition. Analysis made in Hep3B (Control and CRISPR NOX4) cells. A) Cell migration after TGF-β treatment, real-time monitored using xCELLigence system. Results expressed as Normalized Cell Index (left) or slope (h^-1) (right) of the first 16 h. B) Representative images of spheroids from cells either untreated or treated with TGF-β, embedded in a collagen I matrix for 96h. C)SNAI1, SNAI2 and VIM mRNA expression analyzed by RT-qPCR, normalized to housekeeping gene L32, after 48h TGF-β treatment. D) Immunofluorescence of E-Cadherin (green) and DAPI (blue) for nuclei staining after 48h TGF-β treatment. In A-Right and C data are Mean ± SD (n = 3–4). *p<0.05 **p<0.01, ***p<0.001. In B and D, representative images are shown. Scale bar, 50 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)