Fig. 6.

Role of NOX4 on TGF-β-induced cytoskeleton remodeling. Analysis made in Hep3B (Control and CRISPR NOX4) cells. A) Immunofluorescence of Phalloidin (red) and Vinculin (green) after 48h TGF-β treatment. B) Immunofluorescence of Hic-5 (green) after 48h TGF-β treatment. C) Hsp27 and Hic-5 protein levels analyzed by western blot after 48 h of TGF-β treatment. β-Actin was used as loading control D)MMP9 mRNA expression analyzed by RT-qPCR, normalized to housekeeping gene L32, after 48h TGF-β treatment and represented as fold induction (TGF-β-treated versus untreated cells). In A and B, representative images are shown. Scale bar, 25 μm (A). Scale bar, 25 μm (B). In D, data are Mean ± SD (n = 4–6). *p<0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)