Table 5. Optimization of peakcalling with Peakranger.
Number of peaks identified for each condition. ChIPmentation on top. In bold: 1000 bp was selected as the best extension length and applied to N-ChIP data below. N-ChIP A is for 0.8x10 6 cells.
Sample | C1 | C3 | C5 | C7 | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
cell
equivalents |
1,000,000 | 10,000 | 1,000 | 100 | ||||||||
ChIPmentation | ChIPmentation | ChIPmentation | ChIPmentation | |||||||||
Peakranger
read extension length in bp |
L1 | R1 | N-ChIP A | L3 | R3 | N-ChIP B | L5 | R5 | N-ChIP C | L7 | R7 | |
100 | 6,871 | 6,767 | 625 | 424 | 200 | 370 | 4,732 | 5,683 | ||||
300 | 10,369 | 9,545 | 2,125 | 1,211 | 499 | 635 | 3,944 | 5,264 | ||||
600 | 10,581 | 9,924 | 3,513 | 2,823 | 610 | 813 | 3,197 | 3,816 | ||||
1,000 | 9,632 | 9,835 | 8,320 | 4,033 | 2,749 | 3,888 | 695 | 811 | 131 | 3,098 | 3,416 | |
1,500 | 9,901 | 9,426 | 3,315 | 2,925 | 847 | 837 | 4,019 | 3,526 | ||||
2,000 | 9,067 | 8,353 | 3,052 | 2,631 | 626 | 693 | 4,336 | 3,897 |