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. 2023 Jul 13;14:1166951. doi: 10.3389/fimmu.2023.1166951

Figure 2.

Figure 2

Use of scRNA-seq to identify CD8+ TIL subsets in lung tumors from A/J mice. (A, B) Binding assay. Suppression of PD-L1 expression in miR-200c and miR-138-treated mice. Predicted binding sites for miRNA in (A) human and mouse PD-L1; (B) Pdl1 mRNA expression levels were suppressed by miR-138, miR-200c and combo treatment in mouse lung tumor cells. (C) Clustering of intratumoral CD8+ T cells into four Cd8+ TIL subsets; (D) The combination treatment (mir-138-5p and mir-200c-3p) increased the abundance of the anti-tumor EM-like CD8+ T cells and decreased the abundance of the exhausted CD8+ T cells (n = 2; *p< 0.05 and **p< 0.01).