Table 2.
Primer sets for S. Typhimurium colitis-associated transcripts
| Mouse Target Gene | Primer Sequence (5’ to 3’) | Source | |
|---|---|---|---|
| Il1 (IL-1β) | Forward: | 5’-CTCTCCAGCCCAAGCTTCCTTGTGC-3’ | (Perez-Lopez et al. 2019) |
| Reverse: | 5’-GCTCTCATCAGGACAGCCCAGGT-3’ | ||
| Il12a (IL-12a) | Forward: | 5’-GGAAGCACGGCAGCAGAATA-3’ | (Perez-Lopez et al. 2019) |
| Reverse: | 5’-AACTTGAGGGGAGAATGAGGAATGG-3’ | ||
| Il17a (IL-17A) | Forward: | 5’-GCTCCAGAAGGCCCTCAGA-3’ | (Perez-Lopez et al. 2019; Godinez et al. 2009) |
| Reverse: | 5’-AGCTTTCCCTCCGCATTGA-3’ | ||
| Il22 (IL-22) | Forward: | 5’-GGCCAGCCTTGCAGATAACA-3’ | (Perez-Lopez et al. 2019; Godinez et al. 2009) |
| Reverse: | 5’-GCTGATGTGACAGGAGCTGA-3’ | ||
| Ifng (IFN-γ) | Forward: | 5’-TCAAGTGGCATAGATGTGGAAGAA-3’ | (Godinez et al. 2009; Deriu et al. 2013) |
| Reverse: | 5’-TGGCTCTGCAGGATTTTCATG-3’ | ||
| Tnf (TNF-α) | Forward: | 5’-CATCTTCTCAAAATTCGAGTGACAA-3’ | (Deriu et al. 2013) |
| Reverse: | 5’-TGGGAGTAGACAAGGTACAACCC-3’ | ||
| Cxcl1 (CXCL1) | Forward: | 5’-TGCACCCAAACCGAAGTCAT-3’ | (Perez-Lopez et al. 2019; Godinez et al. 2009) |
| Reverse: | 5’-TTGTCAGAAGCCAGCGTTCAC-3’ | ||
| Lcn2 (LCN2) | Forward: | 5’-ACATTTGTTCCAAGCTCCAGGGC-3’ | (Deriu et al. 2013) |
| Reverse: | 5’-CATGGCGAACTGGTTGTAGTCCG-3’ | ||
| Actb (β-actin, Housekeeping) | Forward: | 5’-GGCTGTATTCCCCTCCATCG-3’ | (Perez-Lopez et al. 2019) |
| Reverse: | 5’-CCAGTTGGTAACAATGCCATGT-3’ | ||