Skip to main content
. 2023 Jul 8;26(8):107357. doi: 10.1016/j.isci.2023.107357

Figure 3.

Figure 3

The serum progesterone and LH levels after hCG injection within the control and PPOS groups

(A and B) The serum progesterone (A) and endogenous LH levels (B) in the PPOS and control groups after hCG injection (A: n = 8 mice in each group; B: n = 5–6 mice in each group).

(C and D) The LH levels at 0 h before PMSG injection (C, n = 7–12 mice in each group) and 0 h before hCG trigger (D, n = 7–12 mice in each group) in the control and PPOS groups.

(E) Immunofluorescence images of LHCGR expression on the ovaries in the PPOS and control groups when giving hCG injection. (n = 3–5 mice in each group). The preovulatory follicles were indicated by asterisks and one of them indicated by dotted rectangle box was enlarged in the bottom (Scale bar: 500 μm in the upper panel, 100 μm in the middle panel, 50 μm in the bottom panel).

(F) The relative LHCGR fluorescence intensity within control and PPOS groups analyzed by ImageJ software (n = 5 preovulatory follicles within 3–5 mice in each group).

Statistical analysis was performed by two-way repeated ANOVA with Holm-Sidak test in A-B and Mann-Whitney test in C, D, and F. Data are represented as mean ± SEM. ns, no significance (p ≥ 0.05). ∗p < 0.05. ∗p < 0.01.