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. 2023 Jul 8;26(8):107357. doi: 10.1016/j.isci.2023.107357

Figure 4.

Figure 4

The decreased mRNA levels of progesterone synthesis enzymes and PGR expression in the mice of PPOS group

(A–D) Star (A, n = 9–10 mice in each group), Cyp11a (B, n = 9–10 mice in each group), Hsd3B2 (C, n = 10 mice in each group), and Cyp19a (D, n = 7–10 mice in each group) relative mRNA levels in the PPOS and control groups at 4 h after hCG injection.

(E) Immunofluorescence images of PGR expression on the preovulatory follicles in the PPOS and control groups at 6 h after hCG injection (n = 3–5 mice in each group). Scale bar, 50 μm.

(F) The relative PGR fluorescence intensity within control and PPOS groups analyzed by ImageJ software (n = 6 preovulatory follicles within 3–5 mice in each group).

(G) PGR relative mRNA expression levels at 4 h after hCG injection within two groups (n = 7–9 mice in each group).

Statistical analysis was performed by Mann-Whitney test in A–D, F-G. Data are represented as mean ± SEM. ns, no significance (p ≥ 0.05). ∗p < 0.05, ∗∗p < 0.01.