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. 2023 Jun 17;299(8):104939. doi: 10.1016/j.jbc.2023.104939

Figure 3.

Figure 3

Deg1∗-Sec62 degradation is sensitive to perturbation of lipid biosynthesis. Yeast of the indicated genotypes were transformed with a plasmid encoding Deg1∗-Sec62 or an empty vector and subjected to cycloheximide chase and western blot analysis to detect Deg1∗-Sec62 and Pgk1. Means of percent Deg1∗-Sec62 remaining for 3 to 5 biological replicates are plotted. Error bars represent the standard error of the mean. For the experiment depicted in (A), the final three lanes represent yeast supplemented with 500 μM inositol, 2 mM ethanolamine, and 2 mM choline from inoculation through cell harvest and cycloheximide chase. Means of percent Deg1∗-Sec62 remaining 60 min in (A) were evaluated by one-way ANOVA followed by Tukey’s multiple comparison test. Means of percent remaining at indicated times in (B–D) were evaluated by one-way ANOVA followed by Holm-Šídák multiple comparison tests (only pairs relative to wild-type yeast were compared). Means of percent remaining at 60 min in (E) were evaluated by an unpaired, two-tailed t test.