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. 2023 Jun 17;299(8):104939. doi: 10.1016/j.jbc.2023.104939

Figure 4.

Figure 4

INO4 deletion impairs ERAD of Hrd1 and Doa10 substrates.A, ERAD substrates of Hrd1 and Doa10 analyzed in this figure. B–E, yeast of the indicated genotypes were transformed with a plasmid encoding indicated ERAD substrates or an empty vector and subjected to cycloheximide chase and western blot analysis to detect the ERAD substrate and Pgk1. Means of percent ERAD substrate remaining for 3 to 6 biological replicates are plotted. Error bars represent standard error of the mean. Means of percentage of ERAD substrate remaining at 60 min were evaluated by one-way ANOVA followed by Holm-Šídák multiple comparison tests (only pairs relative to wild-type yeast were compared). F, Flag; ProtA, Protein A; Ub, ubiquitin.