Figure 5.

INO4 deletion does not impair degradation of a soluble, nucleoplasmic substrate of soluble ubiquitin ligase Slx5/Slx8. Yeast of the indicated genotypes were transformed with a plasmid encoding α2∗-Ura3-3HA (α2∗-UH) or an empty vector and subjected to cycloheximide chase and western blot analysis of α2∗-UH and Pgk1. Means of percent α2∗-UH remaining for four biological replicates are plotted. Error bars represent standard error of the mean. Means of percent α2∗-UH remaining at 60 min were evaluated by one-way ANOVA followed by Holm-Šídák multiple comparison tests (only pairs relative to wild-type yeast were compared).