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. 2023 Jun 17;299(8):104939. doi: 10.1016/j.jbc.2023.104939

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© 2023 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Loss of INO4 reduces Ubc7-2HA abundance.Left, Yeast of the indicated genotypes were transformed with a plasmid encoding Hrd1-3HA, Ubc7-2HA, Cue1-HA, or an empty vector, harvested, lysed, and subjected to anti-HA and anti-Pgk1 western blotting. Means of steady state abundance for 3 to 4 biological replicates are plotted. Error bars represent the standard error of the mean. Means in (A and C) were evaluated by an unpaired, two-tailed t test. Means in (B) were evaluated by one-way ANOVA followed by a Holm-Šídák multiple comparison test (only pairs relative to wild-type yeast were compared).