Figure 7.

INO4 deletion does not broadly impair translocation.A, electrophoretic migration of plasmid-encoded Deg1∗-Sec62 in yeast of the indicated genotypes was assessed by western blotting. B, top, Electrophoretic migration of plasmid-encoded CPY (a model post-translationally translocated protein) or OPY (a model co-translationally translocated protein) in yeast of the indicated genotypes was assessed by western blotting. Bottom, Means of the proportion of CPY or OPY that is unglycosylated (i.e., untranslocated) for three biological replicates are plotted. Error bars represent standard error of the mean. Means were evaluated by one-way ANOVA followed by Tukey’s multiple comparison test. C, lysates from ino4Δ yeast expressing Deg1∗-Sec62, CPY, or OPY were incubated in the presence or absence of Endoglycosidase H (Endo H) prior to western blotting.