Table 4.
Plasmids used in this study
| Name | Alias | Yeast selection marker | Yeast plasmid type | Description | Source |
|---|---|---|---|---|---|
| pHA-Pdr5∗ | pVJ1; pRH2312 | HIS3 | CEN | HA-tagged Pdr5∗; Pdr5∗ = C1427Y | (19) |
| YCp50-PPRC1-CPY∗-HA | pVJ2; pDN431 | URA3 | CEN | HA-tagged CPY∗ driven by native promoter; CPY∗ = G255R | (108) |
| pRS313 | pVJ26 | HIS3 | CEN | Empty vector | (109) |
| pRS316 | pVJ27 | URA3 | CEN | Empty vector | (109) |
| pRS315 | pVJ40 | LEU2 | CEN | Empty vector | (109) |
| p414-PMET25 | pVJ121 | TRP1 | CEN | Empty vector with MET25 promoter | (110) |
| p416-Deg1-GFP2 | pVJ205; pUL28 | URA3 | CEN | Deg1 fused to two copies of GFP | (49) |
| p416-PMET25-Deg1∗-Sec62-2xProtA | pVJ317 | URA3 | CEN | Deg1∗-Flag-Sec62-2xProtA (“Deg1∗-Sec62”) driven by MET25 promoter; Deg1∗ = F18S, I22T | (14) |
| p416-PGPD-Deg1-Vma12-2xProtA | pVJ343 | URA3 | CEN | Deg1-Flag-Vma12-2xProtA (“Deg1-Vma12”) driven by TDH3 (GPD) promoter | (68) |
| p414-PGAL4-Deg1-Sec62-ProtA-His3 | pVJ477 | TRP1 | CEN | Deg1-Flag-Sec62-2xProtA-His3 (“Deg1-Sec62-His3”) driven by GAL4 promoter | (37) |
| p414-PGAL4-Deg1-Sec62-2xProtA-His3:natmx4 | pVJ490 | TRP1, natMX4 | CEN | Source of PGAL4-Deg1-Sec62-His3:natmX4 cassette to generate query strain for screen; Deg1-Flag-Sec62-2xProtA-His3 (“Deg1-Sec62-His3”) driven by GAL4 promoter; natMX4 is a distinct gene with its own promoter and terminator | This study |
| pRS313-ADH-Ubc7-2HA | pVJ520; STK06-2-5 | HIS3 | CEN | 2HA-tagged Ubc7 driven by ADH promoter | (111) |
| pRS314-UPRE-GFP | pVJ552 | TRP1 | CEN | GFP driven by Unfolded Protein Response Element (UPRE) | (112) |
| pRS316-GPD-CPY-2xProtA | pVJ576 | URA3 | CEN | CPY-2xProtA driven by GPD promoter | (15) |
| pRS316-GPD-OPY-2xProtA | pVJ578 | URA3 | CEN | OPY-2xProtA driven by GPD promoter OPY is CPY with the CPY signal sequence replaced with Ost1 signal sequence (55) | (15) |
| pJM130-MATα2∗-URA3-3HA | pVJ656 | LEU2 | CEN | MATα2 (a substrate of both Doa10 and Slx5/Slx8 ubiquitin ligases) possessing I4T and L10S mutations (which render substrate degradation primarily dependent on Slx5/Slx8) and C-terminal appendage of Ura3 and 3HA epitope | (50) |
| pRS315-Cue1-HA | pVJ667; pGT181 | LEU2 | CEN | HA-tagged Cue1 | (67) |