Figure 4.
Changes in 1H-13C signals of Ile and Phe residues in FliGMC subjected to pressure
(A–E) Overlaid HMQC spectra of FliGMC at 0.1 MPa (black) and 75 MPa (magenta). (B), (C), (D), and (E) highlight the signals from I148; I164; I180, I238, and I249; and I267 residues, respectively, that exhibited a significant change in the chemical shift.
(F and G) Overlaid aromatic CH TROSY HSQC spectra of FliGMC at 0.1 MPa (black) and 75 MPa (magenta). (G) The signal from F256 residue is highlighted. The spectral display threshold for 75 MPa in (E) and (G) is doubled.