Figure 6.

Structural models of the conformational change in the C-ring during switching between CCW and CW states

(A–D) The structural models show CCW (A) and CW (B) states based on the C-ring volumes of the G214S and G215A mutation; the model was generated based on Cryo-ET analysis.⁴⁴ FliG_C, FliG_M FliM and FliN, are shown in light green, light blue, yellow, pink, respectively, as a ribbon model. Side view of FliG_C, FliG_M and FliM from the dotted circle in (A) and (B) are shown in (C) and (D), respectively. The Ile/Phe residues whose NMR signals changed with the G215A mutation or applying pressure to FliG_MC were mapped in the model structure of each FliG by ball representation (red). Among those residues, I197 and F256, which showed particularly large changes, are indicated by blue balls.