KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-mouse CD3 (17A2) Alex488 | BioLegend | 100220; RRID:AB_1732057 |
| Anti-mouse CD8a (53–6.7) BUV395 | BD Biosciences | 563786; RRID:AB_2732919 |
| Anti-mouse CD8a (53–6.7) BV421 | BioLegend | 100738; RRID:AB_11204079 |
| Anti-mouse CD4 (RM4–5) FITC | BioLegend | 100510; RRID:AB_312713 |
| Anti-mouse CD25 (PC61) APC-Cy7 | BioLegend | 102026; RRID:AB_830745 |
| Anti-mouse B220 (RA3–6B2) PE-cy7 | BioLegend | 103222; RRID: AB_313005 |
| Anti-mouse PD-1 (29F.1A12) BV421 | BioLegend | 135218; RRID:AB_2561447 |
| Anti-mouse TIM3 (RMT3–23) APC | BioLegend | 119706; RRID:AB_2561656 |
| Anti-mouse CD45 (30-F11) Percp-cy5.5 | BioLegend | 103132 RRID: AB_893340 |
| Anti-mouse CD45.1 (A20) BV421 | BioLegend | 110732 BRID: AB_2562563 |
| Anti-mouse CD45.2 (104) BUV737 | BD Biosciences | 612778; RRID:AB_2870107 |
| Anti-mouse CD317 (927) Alex488 | BioLegend | 127012 RRID: AB_1953287 |
| Anti-mouse CD11c (N418) FITC | BioLegend | 117306; RRID:AB_313775 |
| Anti-mouse CD11b (M1/70) APC-Cy7 | BioLegend | 101226; RRID:AB_830642 |
| Anti-mouse CD24 (M1/69) BV711 | BioLegend | 563450; RRID:AB_2738213 |
| Anti-mouse MHC II (M5/114.15.2) BV605 | BioLegend | 107639; RRID:AB_2565894 |
| Anti-mouse F4/80 (T45–2342) BUV395 | BD Biosciences | 565614; RRID:AB_2739304 |
| Anti-mouse CD86 (GL-1) PE-Dazzle 594 | BioLegend | 105042; RRID:AB_2566409 |
| Anti-mouse CD103 (2E7) PE | BioLegend | 121406; RRID:AB_1133989 |
| Anti-mouse CD45.1 (A20) APC | BioLegend | 110714; RRID:AB_313503 |
| Anti-mouse IFN-γ (XMG1.2) PE | BioLegend | 505808; RRID:AB_315402 |
| Anti-mouse TNF-α (MP6-XT22) APC | BioLegend | 506308; AB_315429 |
| Anti-mouse Granzyme B (QA16A02) APC | BioLegend | 372204; RRID:AB_2687028 |
| Anti-mouse FoxP3 (150D) PE | BioLegend | 320007 AB_492981 |
| Anti-mouse PGC-1a (D-5) PE | Santa Cruz | sc-518025 PE |
| Anti-mouse Ki67 (11F6) BV421 | BioLegend | 151208 RRID: AB_2629748 |
| Anti-mouse CD206 PE | BioLegend | 141706 RRID: AB_10895754 |
| 1E4.2.1 anti-Env antibody | Wittrup lab at MIT | N/A |
| Anti-mouse IFN-γ (XMG1.2) | BioXCell | BE0055; RRID:AB_1107694 |
| Anti-mouse TNF-α (XT3.11) | BioXCell | BE0058; RRID:AB_1107764 |
| Anti-mouse CD3ε (2C11) | BioXCell | BE0001–1 BRID:AB_1107634 |
| Anti-mouse CD28 (37.51) | BioXCell | BE0015–1 BRID:AB_1107624 |
| Anti-CD45.1 (A20) | Stem cell Tech | 60117BT |
| Anti-CD45.2 (104) | Stem cell Tech | 60118BT |
| Bacterial and virus strains | ||
| 5-alpha Competent E. coli | New England Biolabs | C2987U |
| Chemicals, peptides and recombinant proteins | ||
| 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide (polyethylene glycol)-2000] | Layson Bio | 100220 |
| DSPE-PEG-FITC | Avanti | 810120 |
| Cyclic-di-GMP | invivogen | tlrl-nacdg |
| Resiquimod | invivogen | tlrl-r848 |
| GolgiPlug™ Protein Transport Inhibitor (containing Brefeldin A) | BD Biosciences | BDB555029 |
| Cell Stimulation Cocktail | eBioscience | 00–4970-93 |
| Protease Inhibitor Cocktail | Roche | 5892970001 |
| Recombinant murine IL-2 | Biolegend | 575408 |
| Recombinant murine IFN-γ | Peprotech | 315–05 |
| DNase I | Sigma Aldrich | 10104159001 |
| Collagenase IV | Worthington | LS004188 |
| CalPhos™ Mammalian Transfection Kit | Takara | 631312 |
| Sytox Red | Thermo Fisher | S34859 |
| Retronectin | Takara | T100B |
| TRIzol™ Reagent | Thermo Fisher | 15596018 |
| iTAg Tetramer/PE – H-2 Kb OVA (SIINFEKL) | MBL international | TB-5001–1 |
| Critical commercial assays | ||
| NucleoSpin® Plasmid | Takara | 740588.250 |
| TrypLE™ Express Enzyme | Thermo Fisher | 12605036 |
| Gibco ACK Lysing Buffer | Thermo Fisher | A10492–01 |
| CellTrace Violet | Thermo Fisher | C34557 |
| LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation | Thermo Fisher | L34966 |
| FITC Annexin V Apoptosis Detection Kit | BD Biosciences | 556547 |
| Fixation/Permeabilization Solution Kit | BD Biosciences | 554714 |
| Foxp3 / Transcription Factor Staining Buffer Set | eBioscience | 00–5523-00 |
| Mouse CD45 microbeads | Miltenyi Biotec | 130–052-301 |
| EasySep™ Mouse CD8+ T Cell Isolation Kit | Stemcell Technologies | 19853 |
| Mouse IFN-γ ELISA kit | R&D systems | DY485 |
| Mouse IL-2 ELISA kit | Invitrogen | 88–7024 |
| Mouse IFN-γ ELISPOT Kit | BD Biosciences | 551083 |
| RNeasy Micro Kit | Qiagen | 74004 |
| T-PERTM | Thermo Fisher | 78510 |
| Proteinase and phosphatase inhibitors | Thermo Fisher | 78442 |
| iScript™ Reverse Transcription Supermix | Biorad | 1708841 |
| LEGENDplex™ assays | BioLegend | 740621 |
| Deposited data | ||
| Bulk RNA-seq data | GEO | GSE211938 |
| sc RNA-seq data | GEO | GSE212453 |
| Experimental Models: Cell Lines | ||
| B16F10 cells | ATCC | CRL-6475; RRID:CVCL_0159 |
| CT-2A cells | T. Seyfried Lab at Boston college | N/A |
| MHCII+ CT-2A cells | Generated in the Irvine lab | N/A |
| mEGFRvIII-CT-2A cells | Generated in the Irvine lab | N/A |
| ZsGreen+ mEGFRvIII-CT-2A cells | Generated in the Irvine lab | N/A |
| mEGFRvIII-CT-2A-OVA cells | Generated in the Irvine lab | N/A |
| 293 phoenix cells | ATCC | CRL-3214 |
| B16F10-OVA cells | G. Dranoff Lab at DFCI | N/A |
| 2C TCR-58−/− T cell hybridoma cells | Birnbaum lab at MIT | N/A |
| 7PPG2 TCR-58−/− T cell hybridoma cells | Birnbaum lab at MIT | N/A |
| MC38 cells | Wittrup lab at MIT | N/A |
| TC-1 cells | ATCC | CRL-2493 |
| TRP1−/− B16F10 cells | Generated in the Irvine lab | N/A |
| Experimental Models: Organism/Strains | ||
| C57BL/6J mice, CD45.2+ | Jackson Laboratory | 000624; RRID:IMSR_JAX:000624 |
| C57BL/6J mice, CD45.1+ | Jackson Laboratory | 002014; RRID:IMSR_JAX:002014 |
| Rag1−/− (B6.129S7-Rag1tm1Mom/J) | Jackson Laboratory | 013755; RRID:IMSR_JAX:013755 |
| IFNGR1−/− (B6.129S7-Ifngr1tm1Agt/J) | Jackson Laboratory | 003288; RRID:IMSR_JAX:00328 |
| Batf3−/− (B6.129S(C)-Batf3tm1Kmm/J) | Jackson Laboratory | 013755; RRID:IMSR_JAX:013755 |
| CD11c-cre (C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J ) | Jackson Laboratory | 007567; RRID:IMSR_JAX:007567 |
| IFNGR1-flox (C57BL/6N-Ifngr1tm1.1Rds/J) | Jackson Laboratory | 025394; RRID:IMSR_JAX:025394 |
| PGC-1α-flox (B6N.129(FVB)-Ppargc1atm2.1Brsp/J) | Jackson Laboratory | 009666 RRID:IMSR_JAX:009666 |
| LCK-cre (B6.Cg-Tg(Lck-cre)548Jxm/J) | Jackson Laboratory | 003802 RRID:IMSR_JAX:003802 |
| IL12rb2−/− (B6;129S1-Il12rb2tm1Jm/J) | Jackson Laboratory | 003248 RRID:IMSR_JAX:003248 |
| IL12p40−/− (B6.129S1-Il12btm1Jm/J | Jackson Laboratory | 002693 RRID:IMSR_JAX:002693 |
| IFNγ −/− (B6.129S7-Ifngtm1Ts/J) | Jackson Laboratory | 002287; RRID:IMSR_JAX:002287 |
| CD8α −/− (B6.129S2-Cd8atm1Mak/J) | Jackson Laboratory | 002665 RRID:IMSR_JAX:002665 |
| Oligonucleotides | ||
| Ccl4 qPCR primers For: CCAAGCCAGCTGTGGTATTCC Rev: GAGCTGCTCAGTTCAACTCC |
Sigma-Aldrich | N/A |
| Ccl5 qPCR primers For: GCTGCTTTGCCTACCTCTCC Rev: TCGAGTGACAAACACGACTGC |
Sigma-Aldrich | N/A |
| Itgb1 qPCR primers For: ATGCCAAATCTTGCGGAGAAT Rev: TTTGCTGCGATTGGTGACATT |
Sigma-Aldrich | N/A |
| Itga4 qPCR primers For: GATGCTGTTGTTGTACTTCGGG Rev: ACCACTGAGGCATTAGAGAGC |
Sigma-Aldrich | N/A |
| Cx3cr1 qPCR primers For: CCCATCTGCTCAGGACCTC Rev: ATGGTTCCAAAGGCCACAATG |
Sigma-Aldrich | N/A |