Figure 3.

LONG R³rhIGF1 treatment restored reduced food intake, impaired body weight gain, and reduced gastric compliance of klotho mice. (A) Restoration of reduced food intake (left panel) and body weight gain (middle and right panels) in klotho mice by LONG R³ rhIGF1 treatment (n = 42–44/group). (B) Kaplan–Meier curves showing overall survival indicated in mice. Note the significantly improved lifespan of klotho mice by IGF1 treatment (P = .0013). Statistical significance of survival curves was analyzed with the log-rank test. (C) Reduced gastric compliance of intact stomachs excised from vehicle- or IGF1-treated klotho mice and age-matched WT mice (n = 8–10/group). Stomachs were inflated with 1 mL Krebs solution at 37°C at a rate of 0.1 mL/min while recording luminal pressure for 10 minutes. After a 10-minute infusion, the infusion was stopped and recorded for an additional 5 minutes. Note significantly improved reduced compliance of klotho mice by LONG R³ rhIGF1 treatment. Statistical significance was determined using Kruskal–Wallis 1-way analysis of variance (ANOVA on ranks).