Table 1.
Antibodies Used in WB Studies
| Target | Supplier | Host | Clone/ID | Isotype/lot number | Label | Final concentration |
|---|---|---|---|---|---|---|
| ACTB | LI-COR | Mouse mAb | 926-42212 | IgG2b | 1:10,000 | |
| ACTB | LI-COR | Rabbit mAb | 926-42210 | IgG | 1:10,000 | |
| ANO1 | CST (Beverly, MA) | Rabbit mAb | D1M9Q | IgG/1 | 1:2000 | |
| CCND1 | CST | Rabbit pAb | 2922 | 7 | 1:1000 | |
| CDKN1B | BD | Mouse mAb | 57/Kip1/p27 | IgG1 | 0.125 μg/mL | |
| ERK1/2 | CST | Mouse mAb | 3A7 | IgG1 | 1:4000 | |
| ETV1 | Abcam (Cambridge, MA) | Rabbit pAb | Ab81086 | IgG/GR12174-15 | 0.5 μg/mL | |
| IGF1 | CST | Rabbit mAb | 73034 | IgG | 1:2000 | |
| KIT | R&D Systems | Goat pAb | AF1356 | IgG/IEO0217101 | 0.2 μg/mL for mice | |
| KIT | DAKO (Carpinteria, CA) | Rabbit pAb | A4502 | 100428020A | 1:4000 for human beings | |
| KITL | AbD Serotec | Rabbit pAb | AAM50 | IgG | 0.2 μg/mL | |
| P-ERK1/2 (202Y204 and T185/T187) | CST | Rabbit mAb | 197G2 | IgG | 1:1500 | |
| Secondary Ab: anti-rabbit IgG (H+L) | LI-COR | Donkey pAb | #926-32223 | C90821-03 | IRDye 680 | 1:10,000 |
| Secondary Ab: anti-mouse IgG (H+L) | LI-COR | Donkey pAb | #926-32222 | C71204-03 | IRDye 680 | 1:10,000 |
| Secondary Ab: anti-rabbit IgG (H+L) | LI-COR | Donkey pAb | #926-32213 | C70918-03 | IRDye 800CW | 1:10,000 |
| Secondary Ab: anti-goat IgG (H+L) | LI-COR | Donkey pAb | #926-32214 | C80207-07 | IRDye 800CW | 1:10000 |
Ab, antibody; ACTB, actin beta; H+L, highly cross-adsorbed; mAb, monoclonal antibody; pAb, polyclonal antibody; P-ERK1/2, P-extracellular signal-regulated kinase 1/2.