Table 2.
Target | Supplier | Host | Clone/ID | Isotype/lot number | Label | Final concentration |
---|---|---|---|---|---|---|
ANO1 | Abcam | Rabbit pAb | ab53212 | IgG/GP3295656-1 | 1:1500 | |
KIT | R&D Systems | Goat pAb | AF1356 | IgG/IEO0217101 | 0.2 μg/mL | |
Secondary Ab: anti-rabbit IgG | Life Technologies (Grant Island, NY) | Chicken pAb | A21441 | 1697089 | AF488 | 5 μg/mL |
Secondary Ab: anti-goat IgG | Life Technologies | Chicken pAb | A21468 | 2318436 | AF594 | 5 μg/mL |
Ab, antibody; pAb, polyclonal antibody