Table 3.
Antibodies Used for Flow Cytometry Analysis of Cells Freshly Dissociated From Murine Gastric Muscles
| Target | Supplier | Host/source | Clone/ID | Isotype | Label | Final concentration or μg/106 cellsa |
|---|---|---|---|---|---|---|
| CD16/32b | eBioscience (San Diego, CA) | Rat mc | 93 | IgG2a, λ | 1 μg | |
| CD11bc | eBioscience | Rat mc | M1/70 | IgG2b, κ | PE-Cy7 | 0.0312 μg |
| CD45 | eBioscience | Rat mc | 30-F11 | IgG2b, κ | PE-Cy7 | 0.0312 μg |
| F4/80 | eBioscience | Rat mc | BM8 | IgG2a, κ | PE-Cy7 | 0.0625 μg |
| CD44 antigen | BioLegend (San Diego, CA) | Rat mc | IM7 | IgG2b, κ | APC-Cy7 | 0.0625 μg |
| KIT | eBioscience | Rat mc | ACK2 | IgG2b, κ | APC | 5 μg/mL |
| KIT | eBioscience | Rat mc | 2B8 | IgG2b, κ | APC | 0.25 μg |
| CD34 antigen | eBioscience | Rat mc | RAM34 | IgG2a, κ | eFluor 450 or FITC | 0.2 μg |
APC, allophycocyanin; Cd45, protein tyrosine phosphatase, receptor type, C; Cy7, cyanine 7; mc, monoclonal; F4/80, epidermal growth factor (EGF)-like module containing, mucin-like, hormone receptor-like sequence 1; FITC, fluorescein isothiocyanate; PE, phycoerythrin.
a
Amount added to 100 μL staining volume.
b
Cd16: Fc receptor, IgG, low-affinity III; Cd32: Fc receptor, IgG, low-affinity IIb.
c
Cd11b, integrin α M.