Table 4.
Configuration of the Becton Dickinson LSR II Flow Cytometer
| Laser | Excitation wavelength | Dichroic filter | Emission filter, nm (peak/bandwidth) | Detector type | Light scatter or fluorochromes used |
|---|---|---|---|---|---|
| Coherent Sapphire 20 mW (BD biosciences) | 488 | Photodiode | Forward scatter | ||
| 488/10 | PMT | Side scatter | |||
| 505 LP | 530/30 | PMT | FITC | ||
| 550 LP | 575/26 | PMT | PE | ||
| 595 LP | 610/20 | PMT | Unused | ||
| 685 LP | 695/40 | PMT | Beads | ||
| 735 LP | 780/60 | PMT | PE-Cy7 | ||
| Coherent CUBE 100 mW (BD biosciences) |
407 | 450/50 | PMT | eFluor 450 | |
| 505LP | 525/50 | PMT | Unused | ||
| 535 LP | 590/40 | PMT | Unused | ||
| 595 LP | 610/20 | PMT | Unused | ||
| 630 LP | 670/30 | PMT | Unused | ||
| 670 LP | 710/50 | PMT | Unused | ||
| Coherent CUBE 40 mW (BD biosciences) |
640 | 660/20 | PMT | APC | |
| 685 LP | 712/20 | PMT | Beads | ||
| 735 LP | 780/60 | PMT | APC-Cy7 |
APC, allophycocyanin; Cy7, cyanine 7; FITC, fluorescein isothiocyanate; LP, long pass; PE, phycoerythrin; PMT, photomultiplier tube.