Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 May 18;16(3):411–429. doi: 10.1016/j.jcmgh.2023.05.002

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Nox4 is involved in intestinal fibrosis and immune-mediated tissue regeneration. (A) Immunohistochemistry with antibodies against F4/80 (top) indicating M1 macrophages, CD164 (middle) indicating M2 macrophages, and CD127 (bottom) indicating lymphoid cells on DSS-treated WT and Nox4^-/- mouse colon sections. (B) Immunohistochemistry with antibody against Ki-67 indicating proliferating cells. (C) Sirius red staining showing the fibrotic area in red (top). Immunolabeling with antibodies against Tenascin-C indicates fibrosis in the colon tissue (bottom). Each positive staining area was measured by ImageJ and shown as a bar graph. Grey, WT group (N = 3); red, Nox4^-/- group (N = 3). (D) Reverse-transcription quantitative PCR results of proinflammatory-related genes TNF, and IL1b. (E) Reverse-transcription quantitative PCR results of fibrosis-related genes Col1a1, Col3a1, and TNC. Data are expressed as means ± SD. ∗P < .05, ∗∗P < .01, ∗∗∗P < .005, and ∗∗∗∗P < .001 compared with WT. Ki-67, marker of proliferation Ki(Kiel)-67.