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Preparation of PCR mixture
| Components | Reaction | Final concentration |
|---|---|---|
| PCR-grade water | Add to 50 μl | N.A |
| 2X KAPA HiFi HotStart Ready Mix | 25 μl | 1X |
| Forward primer (10 μM) | 1.5 μl | 0.3 μM |
| Reverse primer (10 μM) | 1.5 μl | 0.3 μM |
| Template gDNA | 50 ng | 1 ng/μl |
