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. 2023 Jun 22;299(8):104948. doi: 10.1016/j.jbc.2023.104948

Table 6.

PCR cycling protocol

No. Steps Temperature (°C) Time Cycle
1 Initial denaturation 98 3 min 1
2 Denaturation 98 30 s 33
3 Annealing 53–60 30 s
4 Extension 72 15–60 s/kb
5 Final extension 72 1 min/kb 1