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. 2023 Jun 23;299(8):104954. doi: 10.1016/j.jbc.2023.104954

Figure 3.

Figure 3

Stoichiometry of E6–E7 complex.A, the sedimentation velocity revealed the sedimentation coefficient of MBP-16E6_4C4S-LxxLL (black, named E6), fl-16E7 (blue, named E7), and the 1:1 molar ratio mixture of E6 and E7 (magenta, named E6/E7) calculated with SEDFIT, version 12.52. E6 was monitored at 280 nm, whereas the E7 and E6/E7 were monitored at 495 nm. B, the sedimentation equilibrium of MBP16E6_4C4S-LxxLL (named E6), fl-16E7 (named E7), and the 1:1 molar ratio mixture of E6 and E7 (named E6/E7). C, the amount of free fl-16E7 (named E7) decreased with an increasing amount of MBP16E6_4C4S-LxxLL, and it was not detected at a molar ratio of approximately 1:1. MBP, maltose-binding protein.