Table 1.
MW and sedimentation coefficient of MBP-16E6_4C4S-LxxLL, fl-16E7, and MBP-16E6_4C4S-LxxLL/fl-16E7
Proteins | MW theoretical (kDa) | MWapp (kDa) | Sedimentation coefficient (sapp) | Oligomeric state |
---|---|---|---|---|
MBP-16E6_4C4S-LxxLL | 62 | 63.4 ± 4.9 | 4.30 | Monomer (E61) |
fl-16E7 | 11 | 19.7 ± 2.1 | 1.70 | Dimer (E72) |
MBP-16E6_4C4S-LxxLL/fl-16E7 | 146 | 142.0 ± 6.5 | 6.70 | 2 × E6 + 2 × E7 |
MWapp indicates the calculated MW.