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. 2023 Jul 13;14:1221532. doi: 10.3389/fimmu.2023.1221532

Figure 2.

Figure 2

CAF increase CXCL13 production during T cell activation T cells were activated with anti-CD3/anti-CD28 coated beads for 96 hrs in the presence or absence of CAF as indicated. (A) Frequency of CXCL13+ cells within CD4+ and CD8+ populations of T cells from healthy donors as determined by flow cytometry in the presence of absence of CAF (n=6 across 4 repeat experiments). (B) CXCL13 levels in the supernatant of Healthy donor T cells activated in the presence or absence of CAF (n=7 across three independent experiments). (C) Frequencies of CXCL13+ cells within CD4+ and CD8+ T cell populations from NSCLC-patients activated in the presence or absence of CAF (n=12). (D) CXCL13 production by T cells from NSCLC patients after 96s hrs activation in the presence, or absence of CAF (n=12). (E) Measurement of CXCL13 in the supernatant of 4 CAF lines cultured in the presence of the indicated recombinant cytokines. (F) Levels of CCL22 and CX3CL1 produced during activation of healthy donor T cells in the presence or absence of CAF (n=7 across 3 independent experiment). All statistical test performed arepaired T tests.