Table 2. Oxygenated Coumarins Obtained through Von Pechmann Synthesis (2a–2h) and Chemical Modification on Oxygenated Coumarin Cores (3a–3g) and Inhibition of Taq DNA Polymerase and Cell Line Assays.
a
HClO4, 85 °C, 6 h.
b
H2SO4, 120 °C, 6 h.
c
Methanol, piperidine, reflux 12 h.
d
NBS, AIBN, DCA, reflux 6 h.
e
CaCO3, H2O, dioxane, 80 °C, 24 h.
f
THF, thioacetic acid, DIPEA, rt, 12 h.
g
The IC50 values are the means from at least three independent experiments (n = 3). Inactive at 200 μM (highest concentration tested).
h
The IC50 value is the mean from two experiments (n = 2). Inactive at 20 μM (highest concentrations tested).
i
SI HCT-116 = [IC50(HEK 293)]/[IC50(HCT-116)].