Table 2.
Resting and active membrane properties of NT, Kv12.1-targeted, and Kv12.2-targeted shRNA-expressing SCN neurons during the day and at night
| Firing rate (Hz) | Rin (GΩ) | Vr (mV) | ISI (ms) | ||
|---|---|---|---|---|---|
| Day ZT 7–12 | NT shRNA | 3.9 ± 0.5* n = 24 |
2.1 ± 0.1 n = 23 |
−41.5 ± 0.7** n = 24 |
221 ± 4* n = 24 |
| Kv12.1-targeted shRNA | 3.3 ± 0.5 n = 13 |
1.8 ± 0.2 n = 11 |
−41.5 ± 1.2 n = 13 |
213 ± 9 n = 13 |
|
| Kv12.2-targeted shRNA | 3.8 ± 0.6 n = 18 |
2.3 ± 0.3 n = 18 |
−40.5 ± 0.9 n = 18 |
243 ± 8 n = 18 |
|
| Night ZT 18–24 | NT shRNA | 1.6 ± 0.3* n = 27 |
0.9 ± 0.1* n = 22 |
−45.8 ± 1.0** n = 27 |
366 ± 24* n = 27 |
| Kv12.1-targeted shRNA | 4.7 ± 0.7 n = 13 |
1.6 ± 0.2 n = 9 |
−38.7 ± 1.9 n = 13 |
187 ± 5 n = 13 |
|
| Kv12.2-targeted shRNA | 3.6 ± 0.8 n = 13 |
1.9 ± 0.3 n = 12 |
−42.6 ± 1.5 n = 13 |
234 ± 6 n = 13 |
|
Values are means ± SEM; n = number of cells; Rin = input resistance; Vr = resting membrane potentials; ISI = interspike interval. Values in daytime and nighttime NT shRNA-expressing neurons are significantly (*P = 0.0001; **P < 0.004) different.