Table 2.
Sources and identification of antibodies used
| Antibody | Source | Identifier | Use (concentration) |
|---|---|---|---|
| Mouse anti-actin | Abcam | Cat: 5441 | WB (1:10,000) |
| Mouse anti-gH | Abcam | Cat: 110227 | WB (1:1,000) |
| Rabbit anti-H3 | Abcam | Cat: 1791 | WB (1:20,000) |
| Rabbit anti-H3K27me3 | Cell Signal Technologies | Cat: 9733T | WB/CUT&Tag (1:100) IF (1:500) |
| Rabbit anti-H3K9me3 | Abcam | Cat: 8898 | WB (1:1,000) |
| Rabbit anti-H4 | Abcam | Cat: 10158 | WB (1:1,000) |
| Mouse anti-ICP0 | Santa Cruz Biotechnology | Cat: 53070 | WB (1:1,000) |
| Mouse anti-ICP8 | Abcam | Cat: 20194 | IF (1:500) |
| Chicken anti-UL34 | Kind gift of Richard Roller, University of Iowa, Iowa City, IA, USA | N/A | WB (1:1,000) IF (1:500) |
| Rabbit anti-macroH2A1 | Abcam | Cat: 37264 | WB (1:1,000) IF (1:500) CUT&Tag (1:100) |
| Rabbit anti-macroH2A1.1 | Cell Signaling Technology | Cat: 12455S | WB (1:1,000) |
| Mouse anti-macroH2A1.2 | Cell Signaling Technology | Cat: 4827S | WB (1:1,000) |
| Rabbit anti-macroH2A2 | Active Motif | Cat: 39874 | WB (1:1,000) |
| HRP-conjugated anti-VP16 | Santa Cruz Biotechnology | Cat. 7546 | WB (1:1,000) |
| Peroxidase-AffiniPure goat anti-mouse | Jackson Immunoresearch Laboratories | Cat: 115-035-003 | WB (1:5,000) |
| Peroxidase-AffiniPure goat anti-rabbit | Jackson Immunoresearch Laboratories | Cat: 111-035-045 | WB (1:5,000) |
| Rabbit anti-mouse IgG | Abcam | Cat: 46540 | CUT&Tag (1:100) |
| Guinea pig anti-rabbit IgG | Antibodies online | Cat: AB1N101961 | CUT&Tag (1:100) |
| Goat anti-rabbit IgG (H+L) AlexaFluor 568 | Thermo Fisher Scientific | Cat: A-11011 | IF (1:300) |
| Goat anti-mouse IgG (H+L) AlexaFluor 488 | Thermo Fisher Scientific | Cat: A-11001 | IF (1:300) |