Table 3.

Primers used for knockout and ddPCR

CRISPR MacroH2A1 gRNA	5′-CCT​CAA​TAG​CAA​GCC​ATC​CTG​T-3′
HSV1_gB_qPCR_F1	5′-CCG​TCA​GCA​CCT​TCA​TCG​A-3′
HSV1_gB_qPCR_R1	5′-CGC​TGG​ACC​TCC​GTG​TAG​TC-3′
HSV1_gB_probe1	6FAM-5′-CCACGAGATCAAGGACAGCGGCC-3′-BHQ1
beta-globin_qPCR_F1	5′-TGA​AGG​CTC​ATG​GCA​AGA​AA-3′
beta-globin_qPCR_R1	5′-GCT​CAC​TCA​GTG​TGG​CAA​AGG-3′
beta-globin_probe2_hex	5HEX/5′-TCCAGGTGAGCCAGGCCATCACTA-3′/3BHQ_1