Precisely timed regulation of enhancer activity defines the binary expression pattern of Fushi tarazu in the Drosophila embryo

. 2023 Jul 24;33(14):2839–2850.e7. doi: 10.1016/j.cub.2023.04.005

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Experimental models: Organisms/strains

D. melanogaster: y[1] w[1118]; +; P{w[+mC]=vasa:eGFP }1,	Bothma et al.²¹	N/A
D. melanogaster: y[1] w[1118]; +; P{w[+mC]=nos:MCP-mCherry-NLS}	Bothma et al.²¹	N/A
D. melanogaster: y[1] w[1118]; +; P{w[+mC]=nos:tdTomatoPCP-NLS}	Fukaya et al.³⁴	N/A
D. melanogaster: y[1] w[1118]; +; Ftz-LlamaTag	This study	N/A
D. melanogaster: y[1] w[1118]; en-PP7; +	This study	N/A
D. melanogaster: y[1] w[1118]; +; PBac{y[+mDint2] w[+mC]= Autoregulatory Enhancer:Ftz-MS2}VK00033	This study	N/A
D. melanogaster: y[1] w[1118]; +; PBac{y[+mDint2] w[+mC]=Zebra Enhancer:Ftz-MS2}VK00033	This study	N/A
D. melanogaster: y[1] w[1118]; +; Ftz-MS2	Lim et al.²⁴	N/A
D. melanogaster: y[1] w[1118]; +; PBac{y[+mDint2] w[+mC]=Hsp70:FtzCDS-LlamaTag}VK00033	This study	N/A

Recombinant DNA

See online repository for plasmid sequences	This study	https://benchling.com/bothmahubrecht/f_/1bKKlptD-ftz/

Software and algorithms

Matlab	Mathworks	https://nl.mathworks.com/products/matlab.html
ImageJ/Fiji	Schindelin et al.³⁵	https://fiji.sc/
TrackMate	Tinevez et al.³⁶	https://imagej.net/plugins/trackmate/
Veed video compression	Veed.io	https://www.veed.io/tools/video-compressor
Drosophila Embryo Image Analysis Pipeline	This study and Bothma et al.²¹	Zenodo: https://doi.org/10.5281/zenodo.7787495