TABLE 3.

PA substitutions that emerged during replication of the WT:MUT mixtures in NHBE cells

Variant	WT:MUT ratio (%)	hpi or passage no.	Additional PA substitution	Frequency (%) by NGS
IAV I38L	0:100	48 hpi	D394N	31
	0:100	72 hpi	D394N	36
	0:100	P1	D394N	27
	0:100	P2	D394N	28
	0:100	P3	D394N	33
	10:90	72 hpi	D394N	20
	10:90	P1	D394N	26
	10:90	P2	D394N	27
	10:90	P3	D394N	30
	25:75	72 hpi	D394N	9
	25:75	P1	D394N	22
	25:75	P2	D394N	22
	25:75	P3	D394N	24
	50:50	P1	D394N	11
	50:50	P2	D394N	11
	50:50	P3	D394N	12
	75:25	P2	D394N	10
	75:25	P3	D394N	10
IBV I38T	0:100	72 hpi	E329G	27
	0:100	P1	E329G	26
	0:100	P2	E329G	37
	0:100	P3	E329G	41
	10:90	72	E329G	21
	10:90	P1	E329G	24
	10:90	P2	E329G	31
	10:90	P3	E329G	38
	25:75	72 hpi	E329G	17