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. 2023 Jun 13;97(7):e00652-23. doi: 10.1128/jvi.00652-23

FIG 8.

FIG 8

Analysis of the role of the four Argonaute proteins in HIV-induced cytotoxicity. (A to E) Confluency over time of HCT116 parental cells or k.o. clones uninfected or infected with 1% (A to C and E) or 2.5% (D) VSV-G pseudotyped HIV supernatant. The VSV-G pseudotyped HIV supernatant batch used in D had lower activity. (F) Western blotting of HCT116 parental and Ago1,2,3-TKO cells. Ago4 expression was quantified by densitometry relative to β-actin. (G) Real-time PCR analysis of Ago4 mRNA relative to GAPDH. (H) Real-time PCR analysis of Ago4 mRNA relative to GAPDH in HCT116 parental or Ago1,2,3-TKO cells 48 h after transfection with a control SmartPool (siNT) or a SmartPool specific for Ago4. Student’s t test P values are displayed.