FIG 1.

Oas1b restricts the pathogenesis of diverse neuroinvasive flaviviruses. (A–D). Five to six-week-old male and female CC019, CC019-Oas1b^del, and C57BL/6J mice were infected with 100 FFU of POWV strain LB (A), POWV DTV strain Spooner (B), WNV strain NY2000 (C), or JEV strain Nakayama (D) via subcutaneous inoculation in the footpad. Lethality was monitored for 21 days. The data are combined from 4 to 5 experiments per virus. E and F. Mouse embryo fibroblasts (MEFs) were harvested from the indicated mouse lines for multistep growth curve analysis. MEFs were infected at a MOI of 0.01 with POWV strain LB (E) or WNV strain NY2000 (F). Supernatants were collected at 4, 24, 48, or 72 h postinfection and titered via focus-forming assay on Vero cells. The results shown are the mean ± SEM of two to three independent experiments that were performed in duplicate or triplicate. Asterisks represent statistical significance (*, P < 0.05; **, P < 0.01; ***, P < 0.001) via a two-way analysis of variance (ANOVA) compared to CC019-Oas1b^del. (G and H). 9 to 12-week-old C57BL/6J wild-type or Oas1g^−/− male and female mice were infected with 100 FFU of POWV strain MB5/12 (G) or WNV strain NY2000 (H) via subcutaneous inoculation in the footpad. Lethality was monitored for 21 days.