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The effect of the incubation of NCI-H460 (drug-sensitive) and NCI-H460/MX20 (drug resistant) cancer cells with 1 μM of MK-2206, for 0, 24, 48 or 72 h, on the intracellular distribution of the ABCG2 transporter, using immunofluorescence. Nuclei were stained with propidium iodide (PI). The merge images represent the combination of an image with PI and ABCG2 were merged into a single image. Scale bar = 25 μm.
