Table 2. Phosphorylation changes after VS formation.
| Enzyme1 | Regulatory sites2 | ||
|---|---|---|---|
| Kinases | 5 and 60 min | 5 min | 60 min |
| AAK1 | S678 | T389 | |
| AKT1 | S473,S477 | ||
| AKT2 | S474,S478 | ||
| BAZ1B | S189,S347 | S312,S349,S947 | |
| BMP2K | S1107, S1111 | ||
| CDK9 | S464 | T303, | |
| CDK11B | S81 | S178 | |
| DYRK1A | S529 | S758 | |
| EEF2K | S462 | S445 | S470,S474 |
| GTF2F1 | T331 | S433 | |
| LCK | Y424 | ||
| MAP3K2 | S153 | ||
| MAP4K4 | S680,S886,S889,S934 | S549 | |
| MAPK14 | T180 | ||
| MARK3 | T530 | ||
| NEK1 | T344 | ||
| NEK4 | S661 | ||
| PAK4 | S104 | S99, S148 | |
| SIK3 | T463 | S668 | |
| TTN | - | - | - |
| ULK1 | S605 | ||
| WEE1 | S150 | T187,T190 | |
| WNK1 | S378 | T60 | |
| ZAP70 | Y493 | ||
| Phosphatases | |||
| DUSP3 | - | - | - |
| INPP5F | S935 | ||
| PPM1H | T113 | S124 | |
| PTPN1 | S50,S378 | ||
| PTPN7 | S149 | S248 | |
| PTPN18 | T409 | ||
| PTPRC | S1009 | S975 | |
| SSH2 | S1286 | T1449 | |
1Cellular kinases and phophatases with phosphosites significantly modulated (fold change ≥ 1.5 and q-value ≤ 0.1)
2Bolded sites were previously identified, unbolded sites were identified in this study. Changes observed at 5, 60, or both are indicated. Dashes (DUSP3) indicates changes in protein level but no change in phosphorylation state.