KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-mouse synaptophysin (1:300) | ProteinTech | Cat# 17785-1-AP; RRID: AB_2271365 |
| Mouse anti-mouse tyrosine hydroxylase (1:200) | EMD Millipore | Cat# MAB318; RRID: AB_2201528 |
| Alexa Fluor 488 donkey anti-rabbit (1:400) | Life Technologies | Cat# A21441; RRID: AB_2535859 |
| Alexa Fluor 594 goat anti-rabbit (1:400) | Life Technologies | Cat# A11072; RRID: AB_142057 |
| Alexa Fluor 488 donkey anti-mouse (1:400) | Life Technologies | Cat# A21202; RRID: AB_141607 |
| Alexa Fluor 594 goat anti-mouse (1:400) | Life Technologies | Cat# A11037; RRID: AB_2534095 |
| Alexa Fluor 647 Goat anti-mouse (1:400) | Life Technologies | Cat# A21235; RRID: AB_2535804 |
| Anti-mouse NEGR1 (1:200) | ProteinTech | Cat# 13674-1-AP; RRID: AB_2877969 |
| Biological samples | ||
| Human subcutaneous abdominal and gluteal white adipose tissue | This study | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| 1uM insulin | Sigma | Cat# 11376497001 |
| 250nM Dexamethasone | Sigma-Aldrich | Cat# D1756 |
| 1% Pen/Strep | Thermo-Scientific | Cat# 15140122 |
| Direct-zol RNA MiniPrep | Zymo Research | Cat# R2052 |
| Rosiglitazone | Cayman Chemical | Cat# 122320-73-4 |
| High-Capacity Reverse Transcription Kits | Applied Biosystems | Cat# 4368813 |
| Dichloromethane (DCM) | Sigma-Adrich | Cat# 270991 |
| Benzyl ether (DBE) | Sigma-Adrich | Cat# 108014 |
| UltraPure Agarose | Invitrogen | Cat# 16500500 |
| Triton X-100 | Sigma-Adrich | Cat# X100-500ML |
| Tween 20 | Sigma Adrich | Cat# P2287-500ML |
| Critical commercial assays | ||
| Visium Spatial Tissue Optimization Slide & Reagent Kit | 10x Genomics | Cat# PN-1000193 |
| Visium Spatial Gene Expression Solution v1 | 10x Genomics | Cat# PN-1000184 |
| Dual Index Kit TT Set A | 10x Genomics | Cat# PN-1000215 |
| Deposited data | ||
| Raw and processed MS data | This study, ProteomeXchange Data | PXD040894 |
| Raw and processed MS data | This study, ProteomeXchange Data | PXD040894 |
| Visium normalized data | This study, Mendeley Data | https://doi.org/10.17632/r6v7yktxnt.1 |
| Visium raw data and Code for Visium data analyses | This study, Mendeley Data | https://doi.org/10.17632/pktxwdvcbz.1 |
| Proteome and Secretome analysis | Dreyfuss et al.65 | https://github.com/jdreyf/ezlimma |
| Rajbhandari et al.,2019 scRNA-seq data of adipose tissue | Rajbhandari et al.28 | GEO: (GSE133486) |
| Ermont et al.,2022 scRNA-seq data of adipose tissue | Ermont et al.66 | GEO: (GSE176067) |
| Ermont et al.,2022 snRNA-seq data of adipose tissue | Ermont et al.66 | GEO: (GSE176171) |
| Sárvári et al.,2021 snRNA-seq data of adipose tissue | Sárvári et al.30 | GEO: (GSE160729) |
| Takahashi et al.,2019 Human microarray | Takahashi et al.5 | GEO: (GSE116801) |
| Stanford et al.,2015 Mouse microarray | Stanford et al.3 | GEO: (GSE68161) |
| Experimental models: Cell lines | ||
| Primary adipose derived stem cells from mouse iWAT | This study | N/A |
| Experimental models: Organisms/strains | ||
| Mouse: Male C57BL/6NCrl | Charles River Lab | Cat# 027 |
| Mouse: Adipo-PRDM16 KO PRDM16lox/lox | Jackson Laboratory | Cat# 24992 |
| Mouse: Adiponectin-cre | Jackson Laboratory | Cat# 28020 |
| Oligonucleotides | ||
| Total of 50 oligonucleotides | This study | Table S4 |
| Software and algorithms | ||
| CellProfiler 3.0 | Carpenter et al67 | http://cellprofiler.org; RRID: SCR_007358 |
| FIJI macro TWOMBLI | Wershof et al.15 | https://github.com/wershofe/TWOMBLI |
| Fiji v1 | ImageJ | https://imagej.net/software/fiji/; RRID: SCR_002285 |
| Imaris 9.7 | BitPlane | https://imaris.oxinst.com; RRID: SCR_007370 |
| Cytoscape | Shannon et al. 68 | https://cytoscape.org; RRID: SCR_003032 |
| String Database | Szklarczyk et al. 69 | https://string-db.org; RRID: SCR_005223 |
| Metascape | Zhou et al. 32 | https://metascape.org; RRID: SCR_016620 |
| GraphPad Prism v9 | GraphPad | https://www.graphpad.com; RRID: SCR_002798 |
| Loupe Browser version 6.0.0 | 10x Genomics | https://10xgenomics.com; RRID: SCR_018555 |
| R 4.04 | See computational analysis section for R packages used | https://www.r-project.org/about.html |
| Spaceranger-1.3.0 | 10x Genomics | https://www.10xgenomics.com |