Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | C57BL/6J | The Jackson Laboratory | Stock#000664 RRID:MGI:3028467 | |
| Gene (Mus musculus) | C57BL/6NJ-Acod1em1(IMPC)J/J | The Jackson Laboratory | Strain #:029340 RRID:IMSR_JAX:029340 | |
| Cell line (Homo sapiens) |
NCI-H295R | ATCC | CRL-2128 | |
| Chemical compound, drug | Ultrapure LPS, E. coli 0111:B4 | InVivoGen | tlrl-3pelps | For in vivo |
| Chemical compound, drug | Raleukin | MedChemExpress | Art. -Nr.: HY-108841 | |
| Chemical compound, drug | Ultrapure lipopolysaccharide from E. coli K12 | InVivoGen | tlrl-peklps | For in vitro |
| Chemical compound, drug | DMM | Sigma-Aldrich | 136441 | |
| Chemical compound, drug | DES | Sigma-Aldrich | 112402 | |
| Chemical compound, drug | FCCP | Agilent Technologies | Seahorse XFp Cell Mito Stress Test Kit 103010-100 | |
| Chemical Compound, drug | Oligomycin | Agilent Technologies | Seahorse XFp Cell Mito Stress Test Kit 103010-100 | |
| Chemical compound, drug | Enasidenib (AG-221) | Selleckchem | S8205 | |
| Chemical compound, drug | 4-Octyl-itaconate | Cayman Chemical | 25374 | |
| Chemical compound, drug | Trolox | Abcam | ab120747 | |
| Peptide, recombinant protein (human) | IL-1β | PeproTech | 200-01B | |
| Peptide, recombinant protein (mouse) | IL-1β | PeproTech | 211-11B | |
| Peptide, recombinant protein (human) | IL-6 | PeproTech | 200-06 | |
| Peptide, recombinant protein (human) | TNFα | PeproTech | 300-01A | |
| Peptide, recombinant protein (mouse) | ACTH | Sigma-Aldrich | A0298 | |
| Chemical compound, drug | Forskolin | Sigma-Aldrich | F3917 | |
| Transfected construct (human) | siRNA to SDHB (ON-TARGETplus siRNA SMARTpool) |
Dharmacon/Thermo Fisher Scientific | L-011773-02-0005 | |
| Transfected construct (human) | siRNA to DNMT1 (ON-TARGETplus siRNA SMARTpool) |
Dharmacon/Thermo Fisher Scientific | L-004605-00-0005 | |
| Transfected construct (mouse) | siRNA to Sdhb (ON-TARGETplus siRNA SMARTpool) |
Dharmacon/Thermo Fisher Scientific | L-042339-01-0005 | |
| Transfected construct (mouse) | siRNA to Dnmt1 (ON-TARGETplus siRNA SMARTpool) |
Dharmacon/Thermo Fisher Scientific | L-056796-01-0005 | |
| Sequence-based reagent | See Table 5 | This paper | qPCR primers | See Table 5 |
| Antibody | Anti-SDHB (Rabbit polyclonal) | Sigma-Aldrich | HPA002868 | 1:1000 for WB 1:300 for IF |
| Antibody | anti-IDH2 (Rabbit polyclonal) | Sigma-Aldrich | HPA007831 | 1:50 for IF |
| Antibody | Anti-DNMT1 (Rabbit monoclonal) | Cell Signaling | #5032 | 1:1000 |
| Antibody | Anti-Tubulin (Mouse monoclonal) | Sigma-Aldrich | T5186 | 1:3000 |
| Antibody | Anti-β-Actin (Rabbit polyclonal) | Cell Signaling | #4967 | 1:1000 |
| Antibody | Anti-SF-1 (Mouse monoclonal) | TransGenic Inc | KO610 | 1:100 |
| Commercial assay or kit | ATP measurement | Abcam | ab83355 | |
| Commercial assay or kit | ATP/ADP measurement | Sigma-Aldrich | MAK135 | |
| Commercial assay or kit | DCFDA/H2DCFDA Cellular ROS Detection Assay Kit | Abcam | ab113851 | |
| Commercial assay or kit | NADP/NADPH Assay | Abcam | ab176724 | |
| Commercial assay or kit | SDH activity | Sigma-Aldrich | MAK197 | |
| Commercial assay or kit | IDH activity | Abcam | ab102528 | |
| Commercial assay or kit | Seahorse XFp Cell Mito Stress Test Kit | Agilent Technologies | 103010-100 | |
| Commercial assay or kit | EZ DNA Methylation Kit | Zymo Research | D5001 | |
| Software, algorithm | ImageJ software | ImageJ (http://imagej.nih.gov/ij/) | RRID:SCR_003070 | |
| Software, algorithm | GraphPad Prism 7.04 software | GraphPad Prism (https://graphpad.com) | RRID:SCR_015807 | |
| Software, algorithm | Morpheus | Broad Institute | https://software.broadinstitute.org/morpheus/ | |
| Software, algorithm | STAR Aligner | Dobin et al., 2013 | ||
| Software, algorithm | Mouse Genome version GRCm38 (release M12 GENCODE) | Anders et al., 2015 | ||
| Software, algorithm | DESeq2_1.8.1 | Anders and Huber, 2010 | ||
| Software, algorithm | ggplot2_1.0.1 | Wickham, 2009 | ||
| Software, algorithm | GSEA | Subramanian et al., 2005 | ||
| Software, algorithm | EGSEA | Alhamdoosh et al., 2017 | ||
| Software, algorithm | Mass Spectrometry Downstream Analysis Pipeline (MS-DAP) (version beta 0.2.5.1) (https://github.com/ftwkoopmans/msdap) | Hondius et al., 2021 | ||
| Software, algorithm | R/Bioconductor, ‘impute’ command running of ‘DEP’ | Zhang et al., 2018 | ||
| Other | TMRE | Thermo Fisher | T669 | 2.5 μM for dissociated adrenocortical cells, 100 nM for NCI-H295R cells |
| Other | Mitotracker Green | Thermo Fisher | M7514 | 0.25 μM for dissociated adrenocortical cells, 100 nM for NCI-H295R cells |
| Other | DAPI stain | Roche, Sigma-Aldrich | 10236276001 | 1:10,000 |
| Other | Lectin Esculentum DyLight488 | Vector Laboratories | DL-1174 | 1:300 |
| Other | 4-Hydroxynonenal | Abcam | ab48506 | 1:200 |