Table 2.

Seedling physiological characteristics under ambient and heat stress conditions.

Treatments	Chlorophyll content (mg/g leaf fresh wt)	Relative water content (%)	Electrolyte leakage (%)
Ambient (28 ± 2°C)
AC	1.71	86.4	47.5
AC + RG	1.97	95.8	33.1
AC + Si	1.80	90.8	41.3
AC + Si + RG	2.22	98.1	31.9
Mean	1.92	92.8	38.4
CD (P < 0.05)	0.04	12.7	3.3
CV (%)	9.1	16.7	10.4
Heat stress (45 ± 2°C)
HS	1.21	71.2	61.1
HS + RG	1.67	89.9	42.6
HS + Si	1.65	82.3	52.3
HS + Si + RG	2.17	92.9	38.7
Mean	1.68	84.1	48.6
CD (P < 0.05)	0.01	7.5	4.4
CV (%)	7.3	11.0	11.4

AC, Seedlings grown in the absence of silicates and bacteria under ambient temperature.

AC + RG, Seedlings co-cultured with Rhizobium sp. IIRR N1 plus G. diazotrophicus bacteria under ambient temperature.

AC + Si, Seedlings co-cultured with solely with insoluble diatomaceous earth and straw under ambient temperature.

AC + Si + RG, Seedlings co-cultured with both silicon and Rhizobium sp. IIRR N1 plus G. diazotrophicus under ambient temperature.

HS, Seedlings grown in the absence of silicates and bacteria under heat stress condition.

HS + RG, Seedlings co-cultured with Rhizobium sp. IIRR N1 plus G. diazotrophicus bacteria under heat stress.

HS + Si, Seedlings co-cultured with solely with insoluble diatomaceous earth and straw under heat stress.

HS + Si + RG, Seedlings co-cultured with both silicon and Rhizobium sp. IIRR N1 plus G. diazotrophicus under heat stress.