Figure 4.

The expression and activation levels of hepatic insulin-SREBP1 signal in gibel carp fed with HCD and NCD during 8 weeks. (a) Represented SREBP1 (green) staining images (scale bar = 20 μm), (b) percentage of nuclear SREBP1 positive hepatocytes, (c) average fluorescence intensity of nuclear SREBP1, (d) expression level of hepatic srebp1, and (e) plasma insulin level. Values are expressed as means ± SEMs, n = 6. Labeled means without a common letter differ among 2 W, 4 W, 6 W, and 8 W (lowercase for NCD, uppercase for HCD), P < 0.05 (one-way ANOVA, Duncan's post hoc test).  ^∗,  ^∗∗,  ^∗∗∗,  ^∗∗∗∗Different from NCD:  ^∗P < 0.05,  ^∗∗P < 0.01,  ^∗∗∗P < 0.005, and  ^∗∗∗∗P < 0.001, NS means no significant difference (two-tailed independent t test).