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. 2023 Jul 27;14(7):475. doi: 10.1038/s41419-023-06008-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A, B Growth curves (day 1–4) and colony formation assays depict the proliferation of H520/H1703 cells treated with shNC or shTHOC3 plus scramble/PFKFB4-OE plasmids. C Transwell assays show migration of shNC/shTHOC3 infected H520/H1703 cells with scramble/PFKFB4-OE (10× magnification). Scale bars = 100 μm. D The glucose uptake, lactic acid and ATP production of shNC/shTHOC3 infected H520/H1703 cells with scramble/PFKFB4-OE after 24 h. E–F Representative images of subcutaneous tumors on the left flank of mice injected with 1 × 10⁶ shNC/shTHOC3 infected H1703 cells with scramble or PFKFB4-OE plasmids (n = 5, each group). The mean weight of each tumor is measured, and tumor growth curves are plotted every 2–3 days. G PFKFB4 mRNA in shNC/shTHOC3 infected H1703 cells with scramble or PFKFB4-OE plasmids was shown by qRT-PCR. H Lactic acid production of the shTHOC3 groups transfected with scramble or PFKFB4-OE plasmids compared to the shNC group. I IHC shows the levels of Ki-67 index, THOC3, and PFKFB4 in three groups (20× and 40× magnification). Scale bars = 100 μm. *P < 0.05; **P < 0.01; ***P < 0.001. Variables are presented as mean ± SD.