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. 2023 Feb 6;44(8):1612–1624. doi: 10.1038/s41401-023-01059-w

Fig. 5. IGF2 promoted macrophage reprogramming in SCNx mice.

Fig. 5

a The qRT-PCR analysis of anti-inflammatory macrophage markers Il-10, Cd206, and Arg1 (n = 6 per group). The macrophages were treated for 48 h with IL-4 (20 ng/mL), LPS (100 ng/mL), and serum collected from Sham-operated or SCNx mice on day 3 post-MI. b The qRT-PCR analysis of M1 macrophage markers Tnf-α, Il-1β, and iNOS (n = 6 per group). c Western blot analysis of CD206, ARG1, IL-1β, and iNOS protein levels in the isolated peritoneal macrophages treated with IL-4 (20 ng/mL), LPS (100 ng/mL), and serum collected from Sham-operated or SCNx mice on day 3 post-MI (n = 3 per group). d Relative densitometric quantification of CD206, ARG1, IL-1β, and iNOS protein levels in the isolated peritoneal macrophages after the specified treatments (n = 3 per group). e The qRT-PCR analysis of Igf2 expression in the LV of Sham-operated and SCNx mice on day 3 post-MI (n = 6 per group). f IGF2 protein concentration in the serum of Sham-operated and SCNx mice on day 3 post-MI (excitation wavelength=450 nm; n = 6 per group). g The qRT-PCR analysis of specific markers in macrophages after the specified treatments (n = 6 per group). h Relative densitometric quantification of CD206 and ARG1 protein levels in IGF2R antibody-untreated or IGF2R antibody-treated macrophages after exposure to SCNx mouse serum (n = 3 per group). i Relative densitometric quantification of CD206 and ARG1 protein levels in the isolated peritoneal macrophages after the specified treatments (n = 3 per group). Data are presented as mean ± SEM; Student’s t-test or one-way ANOVA; *P < 0.05, **P < 0.01, ***P < 0.001, ns not significant.