Skip to main content
. 2023 Mar 7;44(8):1637–1648. doi: 10.1038/s41401-023-01067-w

Fig. 2. Celastrol interacted with Hsp90β by binding to the NBD ATPase pocket.

Fig. 2

a Complex binding model of celastrol (shown in ball-and-stick mode) in the NBD of Hsp90β. Key residues were shown in stick mode and labeled in green. The corresponding residues in NBD of Hsp90α were labeled in orange (PDB code: 3T0Z). b Huh-luc/neo-ET cells were treated with celastrol (Cel), 17-AAG, or Geldanamycin (Geld) for 2 h. The cell pellet was lysed in the presence of these compounds, and then treated with pronase (75 ng/µg of total protein) at RT. The levels of HSP90α and HSP90β were determined by using Western blot analysis. ITC results of the binding between celastrol with (c) HSP90α or (d) HSP90β. e The ATPase activity of HSP90. (f) Heat map generated from real-time PCR data showing mRNA level of heat shock response genes in Huh-luc/neo-ET cells treated with celastrol, Cel-2, and Cel-6 for up to 72 h. g The expression level of HSP90α, HSP90β, HSC70, and HOP with the treatment of 200 nM celastrol, Cel-2, and Cel-6 for up to 72 h. The blot of β-Actin was used as the internal loading control. The results are representative of three separate experiments. (**P < 0.05).