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. 2023 Mar 7;44(8):1637–1648. doi: 10.1038/s41401-023-01067-w

Fig. 4. The anti-HCV activity of celastrol is dependent on the ATPase activity of HSP90β but not HSP90α.

Fig. 4

Huh-luc/neo-ET cells were transiently transfected with HA-HSP90β expression plasmid and treated with celastrol, Cel-2, or Cel-6 for 48 h. The expression levels of (a) HCV RNA and (b) HCV NS proteins were determined by using real-time PCR and Western blot, respectively. Huh-luc/neo-ET cells were transiently transfected with wild-type (WT), A47D, or A47S mutant HA-HSP90β plasmids, and then treated with celastrol for 48 h. The expression levels of (c) HCV RNA and (d) HCV NS3 protein were determined by using real-time PCR and Western blot analysis, respectively. Huh-luc/neo-ET cells were transiently transfected with HA-HSP90α expression plasmid, and treated with different doses of celastrol for 48 h. The expression levels of (e) HCV RNA and (f) HCV NS3 protein were analyzed as in (a) and (b), respectively. Results are representative of three independent experiments. The HCV RNA level and relative band intensity of NS3 protein are presented as mean ± SD (**P < 0.05 compared with untreated control cells or between each other).