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. 2023 Mar 7;44(8):1637–1648. doi: 10.1038/s41401-023-01067-w

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© The Author(s), under exclusive licence to Shanghai Institute of Materia Medica, Chinese Academy of Sciences and Chinese Pharmacological Society 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

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Fig. 6 — a Diagram of the luciferase pIRES2-EGFP vector. b Impact of celastrol, Cel-2, Cel-6, 17-AAG, and CHX on the translation of HCV genotype 1b IRES-dependent reporters in AD293 stable cell line. Impact of celastrol treatment on (c) HCV genotype 2a, (d) MSCV, and (e) CrPV IRES-dependent translation in AD293 cells harboring the pIRES2-EGFP plasmid was analyzed by the ratio of firefly luciferase and EGFP. f Huh-luc/neo-ET cells were transiently transfected with HA-HSP90β for 48 h and then treated with celastrol as indicated. The HA-HSP90β-associated proteins were pulled down with HA magnetic beads and analyzed by using Western blot analysis. Results are representative of three independent experiments. Data in (b–f) were presented as mean ± SD (**P < 0.05 compared with each other or to DMSO treatment).